MUTUAL REPRESSION ENHANCES THE STEEPNESS AND PRECISION OF GENE EXPRESSION BOUNDARIES.


Identify Key Sequence Features to Improve CRISPR sgRNA Efficacy

The CRISPR/Cas9 system is a creative and innovative gene editing biotechnology tool in genetic engineering.Although several achievements have been attained using the CRISPR/Cas9 system, it is still a challenge to avoid off-target effects and improve the editing efficacy.Previous efforts on evaluating the efficacy and designing the guide RNA mainly

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Theory and practice in environmental design: post normal sciences and visioning process oriented design for sustainability

This contribution contains an analysis of the relationship between theory and practice in architecture, started from the particular similarity between scientific and design research and intended to nurture the debate around the possible evolution of the disciplinary tools of Architectural Technology.The contents are organized as follows: In MAGNESI

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Toposes of another world in "the Life of Basil the Younger"

"Life of Basil theYounger," assimilated in the translation of the original Greek into Oral Pain the Church Slavonic language, has had a direct influence on the formation of ancient Russian eschatological concepts, as well as iconographic canons, in particular, on the composition of the B12 FOLATE Last Judgement.His emotional and figurative descript

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